Role of Nitric Oxide Synthesis Inhibitors in Iron-Induced Nigral
Neurotoxicity: A Mechanistic Exploration
Toxicology Mechanisms and Methods, Volume 18, Issue 4 May 2008 , pages
379 - 384
Authors: M. mer Bostanci a; Faruk Bagirici b; Orhan Bas c
Affiliations: a School of Health, Hitit University, Corum/Turkey
b Department of Physiology, Faculty of Medicine, Ondokuz Mayis
University, Samsun/Turkey
c Department of Anatomy, Faculty of Medicine, Afyon Kocatepe
University, Afyon/Turkey
Abstract
In the central nervous system, nitric oxide (NO) has been suggested to
be a cell-to-cell signaling molecule that regulates guanylyl cyclase,
aconitase, and iron regulatory protein.
NO is also one of the substances that is involved in neuronal death.
On the other hand, iron overload and enhanced hydroxyl radical
formation have been implicated as the causative factors of some
neurodegenerative disorders.
The present study was performed to clarify whether nitric oxide is
involved in iron-induced neuron death.
Neurotoxicity was produced by microinjection of iron chloride (200 mM,
2.5 =ECL) into the left cerebral ventricle.
After the intracerebroventricular (ICV) injection, all animals were
kept alive for 10 days.
During this period, animals in the iron + L-NAME (N-nitro-L-arginine
methyl ester) and iron + aminoguanidine groups received
intraperitoneal (IP) L-NAME (30 mg/kg) and aminoguanidine (100 mg/kg)
injections once a day, respectively.
Rats belonging to the control group also received intraperitoneally
the same amount of saline.
After 10 days, the rats were perfused intracardially under deep
urethane anesthesia.
Removed brains were processed using the standard histological
techniques.
The total numbers of neurons in substantia nigra of all rats were
estimated with stereological techniques.
It was found that L-NAME significantly decreased nigral cell loss from
43.2% to 14.0%, while aminoguanidine did not affect cell loss.
Results of the present study suggest that NOS inhibition by L-NAME
seems to have neuroprotective effects on iron-induced nigral
neurotoxicity.
Keywords: Aminoguanidine; Iron; L-NAME; Neurotoxicity; Stereology;
Substantia Nigra
DOI: 10.1080/15376510801891369
Who loves ya.
Tom
Jesus Was A Vegetarian!
http://tinyurl.com/634q5a
Man Is A Herbivore!
http://tinyurl.com/4rq595
DEAD PEOPLE WALKING
http://tinyurl.com/zk9fk
|
