Riboflavin inhibits IL-6 expression and p38 activation in islet
cells.
Cobianchi L, Fornoni A, Pileggi A, Molano RD, Sanabria NY, Gonzalez-
Quintana J, Bocca N, Marzorati S, Zahr E, Hogan AR, Ricordi C,
Inverardi L
Cell Transplant 2008; 17(5):559-66.
Riboflavin is a water-soluble vitamin that reduces the production of
proinflammatory mediators and oxygen radicals.
Because islet beta-cells are very sensitive to oxidative stress and to
cytokines, we investigated the possible cytoprotective effects of
riboflavin on insulinoma NIT-1 cells and on isolated rodent islets.
NIT-1 cells and islets cultured in the presence or absence of 10
microM riboflavin were studied at baseline and after exposure to
cytokines (TNF-alpha, IL-1beta, INF-gamma).
Riboflavin treatment did not affect islet cell viability as *****sed
by flow cytometry for caspases activation.
However, riboflavin prevented the cytokine-induced increase in IL-6
mRNA expression and p38 phosphorylation analyzed by real-time PCR and
immunoassay, respectively. In summary, nontoxic doses of riboflavin
prevent cytokines-induced p38 phosphorylation and IL-6 upregulation in
islet cells.
This observation, together with the safety profile of riboflavin in
the clinical setting, makes it an appealing agent for islet
cytoprotection in islet transplantation protocols.
Cell transplantation [Cell Transplant]
-------------------------------
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