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Thanks very much to Kathy Nye for sending us this article in totality.
Neither Kathy or I were able to successfully OCR it in it's original
format (graphic), so I have retyped it, while trying to keep as much of
it's original appearance as possible.
Once typed this do***ent occupies 8 pages of a Word do***ent. I have
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but am unable to post it in it's entirety here on Newsgroup. The
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intereted in the complete do***ent. I can send it to you via e-mail as
the Word do***ent. Here is the short summary version. .Myrl
In vitro interaction between silicone gel and human
monocyte-macrophages.
Tavazzani F, Xing S, Waddell JE, Smith D, Boynton EL.
Institute of Biomaterials and Biomedical Engineering, University of
Toronto, Toronto, Ontario, Canada.
Controversy remains regarding the ability of silicone materials to
induce a specific immune reaction versus a nonspecific inflammatory
response. Histopathological analysis of the tissue around failed breast
implants reveals chronic inflammation with silicone gel droplets either
surrounded by giant cells or engulfed by macrophages, areas of
fibrosis, and necrosis. Macrophages are the key cells engulfing or
forming foreign body giant cells. To address the mechanisms of
silicone-induced inflammation a model of human monocyte-derived
macrophages (MDMs) was developed. After sonication of silicone gel, the
silicone droplets were embedded in Type I collagen and used to coat
glass coverslips; human MDMs were subsequently seeded on the coverslips
and maintained in culture for up to 7 days. The advantage of the model
was that human macrophages could be studied histologically, and
cytochemically as they interacted with well-characterized silicone
materials. Initial analysis of the human macrophages shows phagocytosis
of the silicone gel within hours of exposure to the material. Analysis
for pro-inflammatory cytokines reveals significant transient secretion
of IL-1 (p < 0.01) over controls by human macrophages upon exposure to
silicone gel at 24 h. Copyright 2004 Wiley Periodicals, Inc.
Key words: in vitro interaction; silicone gel; human cyte-macrophages
***End of Summary. . .
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