"Iron chelating properties of epigallocatechin-3-gallate"
The application of proteomics for studying the neurorescue activity of
the polyphenol (-)-epigallocatechin-3-gallate
Archives of Biochemistry and Biophysics, Volume 476, Issue 2, 15
August 2008, Pages 152-160
Orly Weinreb, Tamar Amit, Moussa B.H. Youdim
Abstract
Ac***ulating evidence suggests that oxidative stress resulting in
reactive oxygen species generation plays a pivotal role in
neurodegenerative diseases, sup****ting the realization of the use of
radical scavengers, metal chelator agents, such as the natural
polyphenols for therapy.
In this study, we have focused on specific identification of proteins
involved in the neurorescue activity of the green tea polyphenol, (-)-
epigallocatechin-3-gallate (EGCG) in a progressive model of neuronal
death, induced by long-term serum deprivation of human neuroblastoma
SH-SY5Y cells.
The study was designed in attempt to define biomarkers for the
mechanism of action of EGCG, associated with its iron chelating
properties and its ability to regulate metabolic energy balance and
affect cell morphology.
By using mass spectrometry analysis combined with gene expression
technique, we have succeeded to identify such genes and proteins (e.g.
ATP synthase mitochondrial F1 complex beta, protein kinase C epsilon,
nerve vascular growth factor inducible precursor and hypoxia inducible
factor-1 alpha).
These results strengthen the notion that the diverse molecular
signaling pathways participating in the neurorescue activity of EGCG
render this multifunctional compound as potential agent to reduce risk
of various neurodegenerative diseases.
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